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slc3a2  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology slc3a2
    Slc3a2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 158 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cd98/CD98+Antibody/pm42822612-72-8-15
    Average 93 stars, based on 158 article reviews
    slc3a2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of T reg Cells.
    Article Snippet: For analysis of surface marker, cells were stained in PBS with antibodies from Biolegend, unless otherwise noted. .. Fluorescence conjugated antibody to anti-CD3, anti-CD4, anti-CD8, anti-CD25, anti-CD39, anti-CD44, anti-CD62L, antiCD73, anti-CTLA-4, anti-ICOS, anti-PD-1, anti-IFN-g, anti-IL-17, anti-IL-4, anti-IL-5, anti-CD28, anti-CD304 (Nrp1), anti-CD357 (GITR), anti-CD45.1, anti-CD45.2, anti-CD45R, anti-CD45RB, anti-CD69, anti-CD71, GARP, Ki-67, LAP, GL7, anti-CD185 (CXCR5), FasL, anti-CD71, anti-CD98, CD36 were from BioLegend; Bad was from Santa Cruz Biotechnology, Bim was from Cell Signaling. .. Anti-Foxp3, Fas were from eBioscience.

    Single Cell:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Clone Assay:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Activity Assay:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Transduction:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Hypoglycemia induces brain metabolic reprogramming and neurodegeneration via serum response factor and myocardin-related transcription factor-A.
    Article Snippet: AntiMAP2 (ab5392; Abcam) and anti-Neurofilament light (NF-L; CH22105, Neuromics, Edina, MN, USA) were used for immunofluorescence staining. .. Anti-Tau (sc-166060), anti-cathepsin D (sc377299), anti-CD98 (sc-59145), all from Santa Cruz Biotechnology Inc), anti-MMP14 MAB918 (R&D Systems, Minneapolis, MN, USA), anti-SLC1A5 (MBS7045915, MyBioSource Inc., San Diego, CA, USA), anti-SLC7A5 (Invitrogen PA5-50485; Thermo Fisher Scientific Inc.), anti-CD68 (76437, Cell Signaling Technology), anti-CD40 (ab13545), anti-CD11b (ab133357), Vimentin (ab92547; all from Abcam), anti-APOE (Invitrogen 701241), anti-iNOS (Invitrogen PA1036) and HRP-linked anti-mouse IgM (Invitrogen 31440; all from Thermo Fisher Scientific Inc.), anti- anti-NFκB (sc-8008), anti- Signal Transduction and Targeted Therapy (2025) 10:412 Collagen I anti-ß-Actin (sc-47778, Santa Cruz), anti-ß-tubulin (sc5274), and anti-Lamin A/C (sc-7292, all from Santa Cruz) Biotechnology Inc.), (NB600-408, Novus), horseradish peroxide (HRP)-linked anti-rabbit IgG (7074, Cell Signaling), and HRP-linked anti-mouse IgG (7076, Cell Signaling Technology) antibodies were used for western blotting. .. Phalloidin tetramethyl rhodamine B isothiocyanate (P1951) and 4,6-diamidino-2-phenylindole (DAPI, D9564; both from Sigma-Aldrich Corp.), Alexa Fluor 488 goat antimouse IgG (Invitrogen A-11001) and Alexa Fluor 594 goat antirabbit IgG (Invitrogen A-11012), and Alexa Fluor 647 goat antichicken IgY (Invitrogen A-21449, Thermo Fisher Scientific Inc.) were used for immunofluorescence staining.

    Staining:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Expressing:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    FACS:

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained for either CD54 or CD98 surface expression as determined by FACS analysis after staining with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5). ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma.
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma
    Article Snippet: .. Single cell clones were tested for Cas9 activity following transduction with sgRNAs for CD54 (ICAM1) or CD98 (SLC3A2) and induction with doxycycline for 10 days, at which point cells were stained with either anti-CD54 (Biolegend, clone HDCD54) or anti-CD98 (Santa Cruz Biotechnologies, clone E-5) and levels of surface expression were determined by FACS analysis. ..

    other:

    Article Title: Polarity protein SCRIB interacts with SLC3A2 to regulate proliferation and tamoxifen resistance in ER+ breast cancer
    Article Snippet: Anti-SCRIB (C-20), anti-SCRIB (C-2), anti-LLGL2 antibody (A-4), anti-CD98 (4F2), anti-CD98 (E-5), anti-Erk2 (C-14), and anti-cyclin D1 (H-295) were purchased from Santa Cruz.

    Article Title: Polarity protein SCRIB interacts with SLC3A2 to regulate proliferation and tamoxifen resistance in ER+ breast cancer.
    Article Snippet: COMMUNICATIONS BIOLOGY | (2022) 5:403 | https://doi.org/10.1038/s42003-022-03363-3 |www.nature.com/commsbio 7 SCRIB (C-20), anti-SCRIB (C-2), anti-LLGL2 antibody (A-4), anti-CD98 (4F2), anti-CD98 (E-5), anti-Erk2 (C-14), and anti-cyclin D1 (H-295) were purchased from Santa Cruz.

    Western Blot:

    Article Title: Hypoglycemia induces brain metabolic reprogramming and neurodegeneration via serum response factor and myocardin-related transcription factor-A
    Article Snippet: Anti-MAP2 (ab5392; Abcam) and anti-Neurofilament light (NF-L; CH22105, Neuromics, Edina, MN, USA) were used for immunofluorescence staining. .. Anti-Tau (sc-166060), anti-cathepsin D (sc-377299), anti-CD98 (sc-59145), all from Santa Cruz Biotechnology Inc), anti-MMP14 MAB918 (R&D Systems, Minneapolis, MN, USA), anti-SLC1A5 (MBS7045915, MyBioSource Inc., San Diego, CA, USA), anti-SLC7A5 (Invitrogen PA5-50485; Thermo Fisher Scientific Inc.), anti-CD68 (76437, Cell Signaling Technology), anti-CD40 (ab13545), anti-CD11b (ab133357), Vimentin (ab92547; all from Abcam), anti-APOE (Invitrogen 701241), anti-iNOS (Invitrogen PA1-036) and HRP-linked anti-mouse IgM (Invitrogen 31440; all from Thermo Fisher Scientific Inc.), anti- anti-NFκB (sc-8008), anti-Collagen I anti-ß-Actin (sc-47778, Santa Cruz), anti-ß-tubulin (sc-5274), and anti-Lamin A/C (sc-7292, all from Santa Cruz) Biotechnology Inc.), (NB600-408, Novus), horseradish peroxide (HRP)-linked anti-rabbit IgG (7074, Cell Signaling), and HRP-linked anti-mouse IgG (7076, Cell Signaling Technology) antibodies were used for western blotting. .. Phalloidin tetramethyl rhodamine B isothiocyanate (P1951) and 4,6-diamidino-2-phenylindole (DAPI, D9564; both from Sigma-Aldrich Corp.), Alexa Fluor 488 goat anti-mouse IgG (Invitrogen A-11001) and Alexa Fluor 594 goat anti-rabbit IgG (Invitrogen A-11012), and Alexa Fluor 647 goat anti-chicken IgY (Invitrogen A-21449, Thermo Fisher Scientific Inc.) were used for immunofluorescence staining.

    Article Title: Hypoglycemia induces brain metabolic reprogramming and neurodegeneration via serum response factor and myocardin-related transcription factor-A.
    Article Snippet: AntiMAP2 (ab5392; Abcam) and anti-Neurofilament light (NF-L; CH22105, Neuromics, Edina, MN, USA) were used for immunofluorescence staining. .. Anti-Tau (sc-166060), anti-cathepsin D (sc377299), anti-CD98 (sc-59145), all from Santa Cruz Biotechnology Inc), anti-MMP14 MAB918 (R&D Systems, Minneapolis, MN, USA), anti-SLC1A5 (MBS7045915, MyBioSource Inc., San Diego, CA, USA), anti-SLC7A5 (Invitrogen PA5-50485; Thermo Fisher Scientific Inc.), anti-CD68 (76437, Cell Signaling Technology), anti-CD40 (ab13545), anti-CD11b (ab133357), Vimentin (ab92547; all from Abcam), anti-APOE (Invitrogen 701241), anti-iNOS (Invitrogen PA1036) and HRP-linked anti-mouse IgM (Invitrogen 31440; all from Thermo Fisher Scientific Inc.), anti- anti-NFκB (sc-8008), anti- Signal Transduction and Targeted Therapy (2025) 10:412 Collagen I anti-ß-Actin (sc-47778, Santa Cruz), anti-ß-tubulin (sc5274), and anti-Lamin A/C (sc-7292, all from Santa Cruz) Biotechnology Inc.), (NB600-408, Novus), horseradish peroxide (HRP)-linked anti-rabbit IgG (7074, Cell Signaling), and HRP-linked anti-mouse IgG (7076, Cell Signaling Technology) antibodies were used for western blotting. .. Phalloidin tetramethyl rhodamine B isothiocyanate (P1951) and 4,6-diamidino-2-phenylindole (DAPI, D9564; both from Sigma-Aldrich Corp.), Alexa Fluor 488 goat antimouse IgG (Invitrogen A-11001) and Alexa Fluor 594 goat antirabbit IgG (Invitrogen A-11012), and Alexa Fluor 647 goat antichicken IgY (Invitrogen A-21449, Thermo Fisher Scientific Inc.) were used for immunofluorescence staining.



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    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of <t>SLC3A2</t> and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
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    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of <t>SLC3A2</t> and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.
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    94
    Novus Biologicals cd98
    <t>CD98</t> is highly expressed across all stages of HCC and is independent of survival and tumor size. ( A ) mRNA expression levels of CD98 in normal liver tissue ( n = 175) versus HCC ( n = 421) patient samples from GTEx and TCGA datasets are shown as box plots. ( B ) From the CLCA cohort obtained via the cBioPortal platform , CD98 mRNA expression in HCC tumors at early stages (BCLC 0 & A, n = 28) versus late stages (BCLC B, n = 81; BCLC C, n = 130) is shown as dot plots. Recurrence-free survival (RFS) of HCC patients with lower CD98 mRNA levels (T1 subgroup, n = 62) compared with those with higher CD98 levels (T3 subgroup, n = 62) was analyzed by log-rank test. ( C ) Scatter plot of CD98 mRNA z-scores showed no significant Pearson correlation with tumor size (cm). ( D ) Representative images of CD98 IHC in HCC TMA (LV1501a) across stages are shown. Scale bars = 100 µm and 50 µm, respectively. IHC staining intensity for CD98 was scored as 0 (negative), 1 (low), 2 (medium), or 3 (high) and plotted. ( E ) Dot plot of CD98 IHC scores. n.s. = not significant, ** = p < 0.01, **** = p < 0.0001 (Student’s t test).
    Cd98, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Journal: Cell Reports Medicine

    Article Title: Multi-omics profiling unveils biological and clinical insights into pulmonary sarcomatoid carcinoma

    doi: 10.1016/j.xcrm.2026.102887

    Figure Lengend Snippet: Derepression of ferroptosis decreases cell proliferation in PSC (A) Western blot showing the knockdown efficiency of SLC3A2 and FTL in PLA-801D cells. (B) The cell proliferation of PLA-801D cells measured using CCK-8 assays following the knockdown of SLC3A2 (left) and FTL (right), respectively. (C) The cell proliferation of PLA-801D cells measured using colony formation assays following the knockdown of SLC3A2 (upper) and FTL (bottom), respectively. Following the knockdown of SLC3A2 or FTL , PLA-801D cells were plated and cultured for 14 days. All colonies were subsequently stained with crystal violet and quantified using ImageJ software. (D) The extracellular GSH concentration of PLA-801D cells was tested following the knockdown of SLC3A2 . (E) Following the knockdown of FTL , PLA-801D cells were stained by FerroOrange and quantified using the microplate reader, respectively. (F) The lysates of PLA-801D cells were immunoblotted with the indicated antibodies following the knockdown of FTL . (G) Following the knockdown of SLC3A2 , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Afterward, the extracellular GSH concentration was tested. (H) Following the knockdown of FTL , PLA-801D cells were treated with 10 μM ferrostatin-1 for 24 h. Then the lysates of PLA-801D cells were immunoblotted with the indicated antibodies. (I) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the cell proliferation of PLA-801D cells was detected by CCK-8 assays, respectively. (J and K) Following the knockdown of SLC3A2 (left) or FTL (right), PLA-801D cells were treated with DMSO or 10 μM ferrostatin-1 for 24 h. Then the fluorescent probes were added into the culture medium to test the relative level of ROS, respectively (J); the cell lysates were collected for bicinchoninic acid assay and the detection of lipid peroxidation MDA (K). (L) The graphic model of the repressed ferroptosis in PSC. NC, negative control; ns, not significant; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001.

    Article Snippet: Cells were lysed with 1% Triton X-100 buffer (Beyotime, ST1723) and then boiled in 5× loading buffer for 10 min. Each sample was electrophoresed on 10% PAGE gels, then transferred to PVDF membranes and immunoblotted with the antibodies targeting SLC3A2 (Abclonal, A24375), FTL (Abclonal, A11241), GPX4 (Abclonal, A1933) and β-Actin (Abclonal, AC004).

    Techniques: Western Blot, Knockdown, CCK-8 Assay, Cell Culture, Staining, Software, Concentration Assay, Acid Assay, Negative Control

    CD98 is highly expressed across all stages of HCC and is independent of survival and tumor size. ( A ) mRNA expression levels of CD98 in normal liver tissue ( n = 175) versus HCC ( n = 421) patient samples from GTEx and TCGA datasets are shown as box plots. ( B ) From the CLCA cohort obtained via the cBioPortal platform , CD98 mRNA expression in HCC tumors at early stages (BCLC 0 & A, n = 28) versus late stages (BCLC B, n = 81; BCLC C, n = 130) is shown as dot plots. Recurrence-free survival (RFS) of HCC patients with lower CD98 mRNA levels (T1 subgroup, n = 62) compared with those with higher CD98 levels (T3 subgroup, n = 62) was analyzed by log-rank test. ( C ) Scatter plot of CD98 mRNA z-scores showed no significant Pearson correlation with tumor size (cm). ( D ) Representative images of CD98 IHC in HCC TMA (LV1501a) across stages are shown. Scale bars = 100 µm and 50 µm, respectively. IHC staining intensity for CD98 was scored as 0 (negative), 1 (low), 2 (medium), or 3 (high) and plotted. ( E ) Dot plot of CD98 IHC scores. n.s. = not significant, ** = p < 0.01, **** = p < 0.0001 (Student’s t test).

    Journal: Cancers

    Article Title: Blu-Ray-Based Quantification of CD98+ Extracellular Vesicles for Early Detection of Hepatocellular Carcinoma

    doi: 10.3390/cancers18071086

    Figure Lengend Snippet: CD98 is highly expressed across all stages of HCC and is independent of survival and tumor size. ( A ) mRNA expression levels of CD98 in normal liver tissue ( n = 175) versus HCC ( n = 421) patient samples from GTEx and TCGA datasets are shown as box plots. ( B ) From the CLCA cohort obtained via the cBioPortal platform , CD98 mRNA expression in HCC tumors at early stages (BCLC 0 & A, n = 28) versus late stages (BCLC B, n = 81; BCLC C, n = 130) is shown as dot plots. Recurrence-free survival (RFS) of HCC patients with lower CD98 mRNA levels (T1 subgroup, n = 62) compared with those with higher CD98 levels (T3 subgroup, n = 62) was analyzed by log-rank test. ( C ) Scatter plot of CD98 mRNA z-scores showed no significant Pearson correlation with tumor size (cm). ( D ) Representative images of CD98 IHC in HCC TMA (LV1501a) across stages are shown. Scale bars = 100 µm and 50 µm, respectively. IHC staining intensity for CD98 was scored as 0 (negative), 1 (low), 2 (medium), or 3 (high) and plotted. ( E ) Dot plot of CD98 IHC scores. n.s. = not significant, ** = p < 0.01, **** = p < 0.0001 (Student’s t test).

    Article Snippet: From the CLCA cohort [ ] via cBioPortal [ ], diagnostic sensitivity (%) of blood AFP (cutoffs: 200 ng/mL and 20 ng/mL) and PIVKA-II (cutoff: 40 mAU/mL) for early (BCLC 0 & A, n = 28) versus late HCC (BCLC B, n = 81; BCLC C, n = 130) were displayed as bar graphs; Figure S2: Comparison of three anti-CD98 antibodies for detecting extracellular CD98 on cells and EVs. (A) Surface CD98 on HepG2 cells quantified using three antibodies (Abcam ab307587, Novus NBP2-36491SS, NHRI#20D5) by flow cytometry.

    Techniques: Expressing, Immunohistochemistry

    CD98 expression is independent of current etiological factors associated with HCC development. ( A , B ) From the CLCA cohort obtained via the cBioPortal platform , CD98 mRNA expression levels in HCC with diverse etiological factors, including fibrosis, cirrhosis, viral infection, alcohol consumption, and smoking history, were compared. Data are shown as dot plots. No statistical significance (n.s.) was observed between groups (Student’s t test).

    Journal: Cancers

    Article Title: Blu-Ray-Based Quantification of CD98+ Extracellular Vesicles for Early Detection of Hepatocellular Carcinoma

    doi: 10.3390/cancers18071086

    Figure Lengend Snippet: CD98 expression is independent of current etiological factors associated with HCC development. ( A , B ) From the CLCA cohort obtained via the cBioPortal platform , CD98 mRNA expression levels in HCC with diverse etiological factors, including fibrosis, cirrhosis, viral infection, alcohol consumption, and smoking history, were compared. Data are shown as dot plots. No statistical significance (n.s.) was observed between groups (Student’s t test).

    Article Snippet: From the CLCA cohort [ ] via cBioPortal [ ], diagnostic sensitivity (%) of blood AFP (cutoffs: 200 ng/mL and 20 ng/mL) and PIVKA-II (cutoff: 40 mAU/mL) for early (BCLC 0 & A, n = 28) versus late HCC (BCLC B, n = 81; BCLC C, n = 130) were displayed as bar graphs; Figure S2: Comparison of three anti-CD98 antibodies for detecting extracellular CD98 on cells and EVs. (A) Surface CD98 on HepG2 cells quantified using three antibodies (Abcam ab307587, Novus NBP2-36491SS, NHRI#20D5) by flow cytometry.

    Techniques: Expressing, Infection

    CD98 is an abundant and detectable EV surface protein in HCC cell lines and patient plasma. ( A ) Protein levels of CD98, five EV markers (CD63, CD9, CD81, Flotillin1, TSG101), and two cellular proteins (Calnexin and β-actin) in HCC cell lines or their secreted EVs from HepG2, Hep3B, and PLC5 cells via ExoQuick-TC kit were analyzed by Western blot. ( B ) Protein levels of CD98, CD63, and ApoA1 with or without EV enrichment via Size Exclusion Chromatography (SEC) in HCC patient plasma were analyzed by Western Blot. ( C ) Illustration of EV morphology of plasma after EV enrichment via SEC from the healthy control and HCC patient using transmission electron microscopy (TEM). Scale bar is 100 nm. The uncropped blots are shown in .

    Journal: Cancers

    Article Title: Blu-Ray-Based Quantification of CD98+ Extracellular Vesicles for Early Detection of Hepatocellular Carcinoma

    doi: 10.3390/cancers18071086

    Figure Lengend Snippet: CD98 is an abundant and detectable EV surface protein in HCC cell lines and patient plasma. ( A ) Protein levels of CD98, five EV markers (CD63, CD9, CD81, Flotillin1, TSG101), and two cellular proteins (Calnexin and β-actin) in HCC cell lines or their secreted EVs from HepG2, Hep3B, and PLC5 cells via ExoQuick-TC kit were analyzed by Western blot. ( B ) Protein levels of CD98, CD63, and ApoA1 with or without EV enrichment via Size Exclusion Chromatography (SEC) in HCC patient plasma were analyzed by Western Blot. ( C ) Illustration of EV morphology of plasma after EV enrichment via SEC from the healthy control and HCC patient using transmission electron microscopy (TEM). Scale bar is 100 nm. The uncropped blots are shown in .

    Article Snippet: From the CLCA cohort [ ] via cBioPortal [ ], diagnostic sensitivity (%) of blood AFP (cutoffs: 200 ng/mL and 20 ng/mL) and PIVKA-II (cutoff: 40 mAU/mL) for early (BCLC 0 & A, n = 28) versus late HCC (BCLC B, n = 81; BCLC C, n = 130) were displayed as bar graphs; Figure S2: Comparison of three anti-CD98 antibodies for detecting extracellular CD98 on cells and EVs. (A) Surface CD98 on HepG2 cells quantified using three antibodies (Abcam ab307587, Novus NBP2-36491SS, NHRI#20D5) by flow cytometry.

    Techniques: Clinical Proteomics, Western Blot, Size-exclusion Chromatography, Control, Transmission Assay, Electron Microscopy

    CD98 co-localizes with classical exosome markers CD63 and CD9 in HepG2-derived EVs. ( A ) Representative images of CD63, CD9, and CD98 surface staining, along with total EV staining by TFP ester–AF488, in HepG2-derived EVs using single-EV imaging at 20× ( upper panel) and 40× ( lower panel). Scale bars = 50 µm and 20 µm, respectively. ( B ) Illustration of the multiplex fluorescence imaging principle of the single-EV imaging technique. ( C ) Line scans (L1–L3) from ( A ) were analyzed by ImageJ 1.54p to display relative fluorescence intensity (RFU) of TFP ester, CD63, CD9, and CD98 per pixel. ( D ) Single-EV profiling of 20× images ( n = 3) quantifying the proportion of positive EVs for each marker (CD63, CD9, and CD98) or marker combination relay on TFP ester–AF488-positive signal (total EVs).

    Journal: Cancers

    Article Title: Blu-Ray-Based Quantification of CD98+ Extracellular Vesicles for Early Detection of Hepatocellular Carcinoma

    doi: 10.3390/cancers18071086

    Figure Lengend Snippet: CD98 co-localizes with classical exosome markers CD63 and CD9 in HepG2-derived EVs. ( A ) Representative images of CD63, CD9, and CD98 surface staining, along with total EV staining by TFP ester–AF488, in HepG2-derived EVs using single-EV imaging at 20× ( upper panel) and 40× ( lower panel). Scale bars = 50 µm and 20 µm, respectively. ( B ) Illustration of the multiplex fluorescence imaging principle of the single-EV imaging technique. ( C ) Line scans (L1–L3) from ( A ) were analyzed by ImageJ 1.54p to display relative fluorescence intensity (RFU) of TFP ester, CD63, CD9, and CD98 per pixel. ( D ) Single-EV profiling of 20× images ( n = 3) quantifying the proportion of positive EVs for each marker (CD63, CD9, and CD98) or marker combination relay on TFP ester–AF488-positive signal (total EVs).

    Article Snippet: From the CLCA cohort [ ] via cBioPortal [ ], diagnostic sensitivity (%) of blood AFP (cutoffs: 200 ng/mL and 20 ng/mL) and PIVKA-II (cutoff: 40 mAU/mL) for early (BCLC 0 & A, n = 28) versus late HCC (BCLC B, n = 81; BCLC C, n = 130) were displayed as bar graphs; Figure S2: Comparison of three anti-CD98 antibodies for detecting extracellular CD98 on cells and EVs. (A) Surface CD98 on HepG2 cells quantified using three antibodies (Abcam ab307587, Novus NBP2-36491SS, NHRI#20D5) by flow cytometry.

    Techniques: Derivative Assay, Staining, Imaging, Multiplex Assay, Fluorescence, Marker

    Identification of plasma CD98+ EVs as a novel diagnostic biomarker for early-stage HCC. ( A ) Schematic illustrating plasma CD98+ EV quantification using ExoCounter technology. ( B ) Original CD98+ EV counts from patient plasma (left) and normalized CD98+ EV index (right) are shown as dot plots. ** = p < 0.01, *** = p < 0.001 (Student’s t test). ( C ) ROC curve showing diagnostic performance of the CD98+ EV index. ( D ) Sensitivity and specificity of the CD98+ EV index (cutoff = 6) were calculated from true/false positive and negative rates in early HCC. AFP (cutoff = 20 ng/mL) was compared as a reference, though AFP data were unavailable for healthy controls. TP = true positive; TN = true negative; FP = false positive; FN = false negative.

    Journal: Cancers

    Article Title: Blu-Ray-Based Quantification of CD98+ Extracellular Vesicles for Early Detection of Hepatocellular Carcinoma

    doi: 10.3390/cancers18071086

    Figure Lengend Snippet: Identification of plasma CD98+ EVs as a novel diagnostic biomarker for early-stage HCC. ( A ) Schematic illustrating plasma CD98+ EV quantification using ExoCounter technology. ( B ) Original CD98+ EV counts from patient plasma (left) and normalized CD98+ EV index (right) are shown as dot plots. ** = p < 0.01, *** = p < 0.001 (Student’s t test). ( C ) ROC curve showing diagnostic performance of the CD98+ EV index. ( D ) Sensitivity and specificity of the CD98+ EV index (cutoff = 6) were calculated from true/false positive and negative rates in early HCC. AFP (cutoff = 20 ng/mL) was compared as a reference, though AFP data were unavailable for healthy controls. TP = true positive; TN = true negative; FP = false positive; FN = false negative.

    Article Snippet: From the CLCA cohort [ ] via cBioPortal [ ], diagnostic sensitivity (%) of blood AFP (cutoffs: 200 ng/mL and 20 ng/mL) and PIVKA-II (cutoff: 40 mAU/mL) for early (BCLC 0 & A, n = 28) versus late HCC (BCLC B, n = 81; BCLC C, n = 130) were displayed as bar graphs; Figure S2: Comparison of three anti-CD98 antibodies for detecting extracellular CD98 on cells and EVs. (A) Surface CD98 on HepG2 cells quantified using three antibodies (Abcam ab307587, Novus NBP2-36491SS, NHRI#20D5) by flow cytometry.

    Techniques: Clinical Proteomics, Diagnostic Assay, Biomarker Discovery

    The CD98+ EV index is an independent and early detection biomarker. ( A ) In the non-viral early HCC cohort, CD98+ EV index values were compared across subgroups with different risk factors, including sex, smoking, alcohol consumption, and cirrhosis. Data are shown as dot plots. No significant differences (n.s.) were observed between groups (Student’s t test). ( B ) Bar graphs show diagnostic sensitivity (%) for blood AFP (200 ng/mL and 20 ng/mL), and CD98+ EV index in early HCC (stage I/II, n = 136) and small tumors (≤3 cm, n = 51). ** = p < 0.01, **** = p < 0.0001 (Z-score test for two proportions).

    Journal: Cancers

    Article Title: Blu-Ray-Based Quantification of CD98+ Extracellular Vesicles for Early Detection of Hepatocellular Carcinoma

    doi: 10.3390/cancers18071086

    Figure Lengend Snippet: The CD98+ EV index is an independent and early detection biomarker. ( A ) In the non-viral early HCC cohort, CD98+ EV index values were compared across subgroups with different risk factors, including sex, smoking, alcohol consumption, and cirrhosis. Data are shown as dot plots. No significant differences (n.s.) were observed between groups (Student’s t test). ( B ) Bar graphs show diagnostic sensitivity (%) for blood AFP (200 ng/mL and 20 ng/mL), and CD98+ EV index in early HCC (stage I/II, n = 136) and small tumors (≤3 cm, n = 51). ** = p < 0.01, **** = p < 0.0001 (Z-score test for two proportions).

    Article Snippet: From the CLCA cohort [ ] via cBioPortal [ ], diagnostic sensitivity (%) of blood AFP (cutoffs: 200 ng/mL and 20 ng/mL) and PIVKA-II (cutoff: 40 mAU/mL) for early (BCLC 0 & A, n = 28) versus late HCC (BCLC B, n = 81; BCLC C, n = 130) were displayed as bar graphs; Figure S2: Comparison of three anti-CD98 antibodies for detecting extracellular CD98 on cells and EVs. (A) Surface CD98 on HepG2 cells quantified using three antibodies (Abcam ab307587, Novus NBP2-36491SS, NHRI#20D5) by flow cytometry.

    Techniques: Biomarker Discovery, Diagnostic Assay